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DNA-encoded Library (DEL)
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DNA-encoded library (DEL) is a technology where each small molecule compound is linked to a unique DNA sequence at the DNA level, enabling DNA encoding similar to assigning a "barcode" to each molecule. DEL screening is a technique used in the early stages of drug discovery to rapidly screen billions of small molecule compounds in order to identify new chemical structures for further validation and optimization.
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Workflow of Solid-phase DEL Screening
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1.
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The target protein (blue), immobilized on solid support, is incubated with DEL.
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2.
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Unbound library members are washed away by means of consecutive washing steps.
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3.
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Library members bound to the target protein are eluted.
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4.
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After final elution, the DNA tags on the eluted protein-binding library members are amplified by PCR.
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5.
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The amplified DNA tags are subjected to second-generation high-throughput sequencing, and the resulting data can be plotted to visualize the final bar-code counts for each DEL member.
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6.
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The eluted library members can be used for a further consecutive selection round against fresh, immobilized target before PCR amplification and sequencing.
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Figure 1. Affinity-based DEL selections against an immobilized target protein[1].
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Analysis of DEL Data
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1.
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Decoding of DNA sequencing data using a data analysis program, and comparing with the corresponding DNA encoding and molecular building blocks during library design, will give the structure of the target compounds.
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2.
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Each axis of the cube graph provides the molecular building blocks used in the library preparation process, with each data point representing a specific enriched library member.
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Figure 2. A cube plot of potential hit molecules[1].
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MedChemExpress (MCE) provides one-stop DEL screening services with
photo-cross-linking DELs, covalent DELs, and cyclic peptide DELs. Moreover, depending on the specific building block structure of your request, customized DEL synthesis services can be provided. Through the use of cutting-edge technologies, MCE’s expert DEL synthesis team have developed more than 100 different types of chemical reactions for the DNA tag.
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MedChemExpress DEL Libraries
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Product Name
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Description
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DEL A Kit
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20 DEL libraries, covering over 300 million DEL molecules, 1 tube set.
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DEL B Kit
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50 DEL libraries, covering over 1 billion DEL molecules,
1 tube set.
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DEL T Kit
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68 DEL libraries, covering over 2 billion DEL molecules,
1 tube set.
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DEL C Kit Covalent Library
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5 DEL libraries, covering over 3 million DEL molecules,
1 tube set.
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DEL D Kit Cyclic Peptide Library
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5 DEL libraries, covering over 8 million DEL molecules,
1 tube set.
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DEL E Kit Regular Library
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5 DEL libraries, covering over 190 million DEL molecules,
1 tube set.
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DEL F Kit Regular Library
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5 DEL libraries, covering over 109 million DEL molecules,
1 tube set.
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DEL G Kit Regular Library
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5 DEL libraries, covering over 113 million DEL molecules,
1 tube set.
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DEL H Kit Regular Library
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10 DEL libraries, covering over 550 million DEL molecules, 1 tube set.
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Reference:
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[1] DNA-encoded chemical libraries. Nat Rev Methods Primers 2022;2:3.
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Products are for research use only and are not intended for human use. We do not sell to patients.
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