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- サプライヤー
- TargteMol
- カタログNo.
- TMPY-01964
- 製品名称
- CD16a Protein, Human, Recombinant (F176V, His)
- タンパク質名
- CD16a
- Species
- Human
- HOST
- HEK293 Cells
- 100μg: -
- お問い合わせ
Overview
| Synonyms | FCGRIII, Fc gamma RIIIa, IGFR3, FCR-10, FCRIII, FCRIIIA, FCGR3, CD16A, Fc fragment of IgG, low affinity IIIa, receptor (CD16a), IMD20, CD16, FCG3, Fc γ RIIIa |
|---|---|
| Characteristics | 1. Immobilized Human FcγRIIIA / CD16a (V176) recombinant protein at 2 μg/mL can bind Human IgG1 , the EC50 is 150-450 ng/mL. 2.Loaded Human FcγRIIIA / CD16a (V176) recombinant protein on His1K Biosensor, can bind Bevacizumab (IgG1) with an affinity constant of 0.83 μM as determined in a BLI assay . 3.Captured Human FcγRIIIA / CD16a (V176) recombinant protein ?on?Anti-His?Chip?can?bind?Bevacizumab (IgG1) with?an?affinity?constant?of 0.31 μM?as?determined?in?an?SPR?assay. 4.Captured? Bevacizumab (IgG1)?on?proA Chip?can?bind? Human FcγRIIIA / CD16a (V176) recombinant protein with?an?affinity?constant?of?0.78 ?M?as?determined?in?an?SPR?assay. |
| Endotoxin Level | < 1.0 EU/μg of the protein as determined by the LAL method. |
| Purity | SDS-PAGE: 96.7%; SEC-HPLC: 99.0% |
| Description | The Fc receptor with low affinity for IgG (FCGR3, or CD16) is encoded by 2 nearly identical genes, FCGR3A and FCGR3B, resulting in tissue-specific expression of alternative membrane-anchored isoforms. FCGR3A, it is also known as CD16a, encodes a transmembrane protein expressed on activated monocytes/macrophages, natural killer (NK) cells, and a subset of T cells. CD16a / FCGR3A is a receptor expressed on NK cells that facilitates antibody dependent cellular cytotoxicity (ADCC) by binding to the Fc portion of various antibodies. CD16a / FCGR3A also has a broader function. CD16a / FCGR3A is directly involved in the lysis of some virus-infected cells and tumor cells by NK cells, independent of antibody binding. Cross-linking of CD16a / FCGR3A on NK cells resulted in increased intracellular Ca2+ levels and a cascade of biochemical events similar to those activated by the T cell receptor. CD16a / FCGR3A on human NK cells is a lysis receptor that mediates the direct killing of some virus infected and tumor cells, independent of antibody ligation. |
| Reference | David Dornan, et al. Effect of FCGR2A and FCGR3A variants on CLL outcome. Blood. 2010 116: 4212-4222 |
| URL | https://www.targetmol.com/recombinant-protein/cd16a_protein_human_recombinant_ecd_f176v_his_tag_hplc_verified |
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