R-Spondin 1/RSPO1 Protein, Human, Recombinant (His) [TMPY-01147]

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Synonyms	R-spondin 1, RSPO, CRISTIN3
Characteristics	1. Measured by its binding ability in a functional ELISA. Immobilized human RSPO1 at 20 μg/ml (100 μl/well) can bind human LIMPII with a linear range of 32-800 ng/ml. 2. Measured by its binding ability in a functional ELISA. Immobilized human RSPO1 at 20 μg/ml (100 μl/well) can bind mouse CD36 with a linear range of 6.4-800 ng/ml. 3. Measured by its ability to induce activation of ?catenin response in a Topflash Luciferase assay using HEK293T human embryonic kidney cells. The ED50 for this effect is typically 0.1-0.9 μg/mL in the presence of 5 ng/mL recombinant mouse Wnt3a.
Endotoxin Level	< 1.0 EU/μg of the protein as determined by the LAL method.
Purity	99.30%
Description	R-Spondin 1/RSPO1 Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 28.2 kDa and the accession number is Q2MKA7-1.
Reference	Kamata T, et al. (2004) R-spondin, a novel gene with thrombospondin type 1 domain, was expressed in the dorsal neural tube and affected in Wnts mutants. Biochim Biophys Acta. 1676(1):51-62.,Ota T, et al. (2004) Complete sequencing and characterization of 21,243 full-length human cDNAs. Nat Genet. 36(1):40-5.,Strausberg RL, et al. (2003) Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. Proc Natl Acad Sci. 99(26):16899-903.
URL	https://www.targetmol.com/recombinant-protein/rspo1_protein_human_recombinant_his_tag_

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