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- サプライヤー
- AcroBioSystems
- カタログNo.
- SPN-H52H5
- 製品名称
- HCoV-HKU1 (isolate N5) Spike Trimer Protein, His Tag (MALS verified)
- タンパク質名
- Spike protein
- Species
- HCoV-HKU1
- HOST
- HEK293
Overview
| Synonyms | - |
|---|---|
| Characteristics | This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 145.5 kDa. The protein migrates as 180-210 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation. |
| Endotoxin Level | 1.0 EU per μg |
| Purity | 95% |
| Description | HCoV-HKU1 (isolate N5) Spike Trimer, His Tag (SPN-H52H5) is the ectodomain of HCoV-HKU1 (isolate N5) spike protein which contains AA Ala 13 - Asn 1276 (Accession # Q0ZME7-1). The recombinant protein is expressed from human 293 cells (HEK293) with T4 fibritin trimerization motif and a polyhistidine tag at the C-terminus. The S1/S2 furin-recognition site 752-RRKRR-756 is mutated to GGSGS to generate the uncleaved protein. Proline substitutions (N1067P, L1068P) are introduced to stabilize the trimeric prefusion state of the spike protein. |
| Reference | - |
| URL | https://www.acrobiosystems.com/P3956-HCoV-HKU1-%28isolate-N5%29-Spike-Trimer-Protein-His-Tag-%28MALS-verified%29.html |
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