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タンパク質カタログ
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- サプライヤー
- AcroBioSystems
- カタログNo.
- SPN-C524x
- 製品名称
- SARS-CoV-2 Spike Trimer Protein, His Tag (BA.2.86/Denmark/EPI_ISL_18097315) (MALS verified)
- タンパク質名
- Spike protein
- Species
- SARS-CoV-2
- HOST
- HEK293
Overview
| Synonyms | - |
|---|---|
| Characteristics | This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 138.0 kDa. The protein migrates as 180.0 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE). |
| Endotoxin Level | 1.0 EU per μg |
| Purity | 90% |
| Description | SARS-CoV-2 Spike Trimer Protein, His Tag (BA.2.86/Denmark/EPI_ISL_18097315) (SPN-C524x) is expressed from human 293 cells (HEK293). It contains AA Val 16 - Pro 1213 (Accession # QHD43416.1 (A27S,S50L,H69del,V70del,V127F,G142D,Y144del,F157S,R158G,N211del,L212I,V213G,L216F,H245N,A264D,I332V,G339H,K356T,S371F,S373P,S375F,T376A,R403K,D405N,R408S,K417N,N440K,V445H,G446S,N450D,L452W,N460K,S477N,T478K,N481K,E484K,F486P,Q498R,N501Y,Y505H,E554K,A570V,D614G,P621S,H655Y,N679K,P681R,N764K,D796Y,S939F,Q954H,N969K,P1143L,R683A, R685A, F817P, A892P, A899P, A942P, K986P, V987P)). The spike mutations are identified on the SARS-CoV-2 Omicron variant (Pango lineage: BA.2.86/Denmark/EPI_ISL_18097315). The recombinant protein is expressed from human 293 cells (HEK293) with T4 fibritin trimerization motif and a polyhistidine tag at the C-terminus. Proline substitutions (F817P, A892P, A899P, A942P, K986P, V987P) and alanine substitutions (R683A and R685A) are introduced to stabilize the trimeric prefusion state of SARS-CoV-2 S protein and abolish the furin cleavage site, respectively. |
| Reference | - |
| URL | https://www.acrobiosystems.com/P6863-SARS-CoV-2-Spike-Trimer-Protein-His-Tag-%28BA286DenmarkEPI%5FISL%5F18097315%29-%28MALS-verified%29.html |
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