| Synonyms |
- |
| Characteristics |
Human Elongin B&Elongin C&VHL Heterotrimer Protein, Strep II Tag&Strep II Tag&His Tag, produced by co-expression of Elongin B and Elongin C and VHL, has a calculated MW of 14.5 kDa (Elongin B) and 13.8 kDa (Elongin C) and 25.8 kDa (VHL). Subunit Elongin B is fused with a Strep II tag at the N-terminus and Elongin C is fused with a Strep II tag at the N-terminus and VHL is fused with a polyhistidine tag at the N-terminus. The protein migrates as 17 kDa (Elongin B) and 14 kDa (Elongin C) and 30 kDa (VHL) under reducing (R) condition (SDS-PAGE) due to glycosylation. |
| Endotoxin Level |
1.0 EU per μg |
| Purity |
95% |
| Description |
Elongin B (ELOB) and Elongin C (ELOC) form a heterodimer that serves as the regulatory subunit for the Elongin complex--a general transcription elongation factor that increases RNA Polymerase II transcription through template-encoded arresting sites. The ELOB/ELOC complex also binds to the "BC-box motif" found in many proteins in the VHL-box and SOCS-box protein families. In this function, ELOB/ELOC serves as an adapter between substrate recognition proteins and either Cullin-2/Rbx1 (in VHL-box E3 Ubiquitin ligases) or Cullin-5/Rbx2 (in SOCS-box E3 Ubiquitin ligases). VHL is involved in the ubiquitination and subsequent proteasomal degradation via the von Hippel-Lindau ubiquitination complex. It seems to act as a target recruitment subunit in the E3 ubiquitin ligase complex and recruits hydroxylated hypoxia-inducible factor (HIF) under normoxic conditions. Involved in transcriptional repression through interaction with HIF1A, HIF1AN and histone deacetylases. |
| Reference |
- |
| URL |
https://www.acrobiosystems.com/P4339-Human-Elongin-BElongin-CVHL-Heterotrimer-Protein-Strep-II-TagStrep-II-TagHis-Tag-%28MALS--SPR-verified%29.html |
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